Decompensate a flowFrame

Reverse the application of a compensation matrix on a flowFrame

# S4 method for flowFrame,matrix
decompensate(x, spillover)

# S4 method for flowFrame,data.frame
decompensate(x, spillover)

Arguments

x

flowFrame.
spillover

matrix or data.frame.

Value

a decompensated flowFrame

Examples

library(flowCore)
f = list.files(system.file("extdata",
   "compdata",
   "data",
   package="flowCore"),
 full.name=TRUE)[1]
f = read.FCS(f)
spill = read.csv(system.file("extdata",
       "compdata", "compmatrix",
        package="flowCore"),
        ,sep="\t",skip=2)
colnames(spill) = gsub("\\.","-",colnames(spill))
f.comp = compensate(f,spill)
f.decomp = decompensate(f.comp,as.matrix(spill))
sum(abs(f@exprs-f.decomp@exprs))
#> [1] 2.811873e-11
all.equal(decompensate(f.comp,spill)@exprs,decompensate(f.comp,as.matrix(spill))@exprs)
#> [1] TRUE
all.equal(f@exprs,decompensate(f.comp,spill)@exprs)
#> [1] TRUE